Applications
| Reactive Species | Human, Mouse, Rat |
| Specificity | Mono-Methylated-Histone H3 (K80) Polyclonal Antibody detects endogenous levels of Histone H3 protein only when mono-Methylatedated at K80. |
| Applications | WB, ELISA |
| Recommended Dilution | WB 1:500-1:2000; ELISA 1:20000; Not yet tested in other applications. |
Product Information
| Product Name | Histone H3 (Mono Methylated Lys80) pAb |
| Antibody Type | Rabbit pAb |
| Immunogen | Synthesized peptide derived from human Histone H3 around the mono-Methylatedation site of K80. |
| Modification | Mono Methylated |
| Host | Rabbit |
| Clonality | Polyclonal |
| Clone No. | N/A |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification Method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage Buffer | PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage Conditions | -15°C to -25°C/1 year (Do not lower than -25°C). |
Background Information
| Function | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015], |
| UniProt ID | P68431; Q71DI3; P84243; Q6NXT2 |
| Organism | Human |
| Recommended Name | Histone H3.1/Histone H3.2/Histone H3.3/Histone H3.3C |
| Short Name | Histone H3 |
| Alternative Names | H3K80ME1; HIST1H3A; H3FA; HIST1H3B; H3FL; HIST1H3C; H3FC; HIST1H3D; H3FB; HIST1H3E; H3FD; HIST1H3F; H3FI; HIST1H3G; H3FH; HIST1H3H; H3FK; HIST1H3I; H3FF; HIST1H3J; H3FJ; Histone H3.1; Histone H3.1; Histone H3.2; Histone H3/m; Histone H3/o; H3F3A; H3.3A; H3F3; PP781; H3F3B; H3.3B; Histone H3.3; H3F3C; Histone H3.3C; Histone H3.5. |
| Gene ID | 8350; 8351; 8352; 8353; 8354; 8355; 8356; 8357; 8358; 8968; 126961; 333932; 653604; 3020; 3021; 440093 |
| Gene Name | HIST1H3A/HIST1H3/HIST1H3C/HIST1H3D/HIST1H3E/HIST1H3F/HIST1H3G/HIST1H3H/HIST1H3I/HIST1H3J/HIST2H3A/HIST2H3C/HIST2H3D/H3F3A/H3F3B/H3F3C |
| Calculated MW | N/A |
| Observed MW | 17 kDa |
| Subcellular Location | Nucleus. Chromosome. |
| Summary | N/A |
| Pathways | N/A |
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Histone H3 (Mono Methylated Lys80) pAb
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