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首页 抗体 一抗 KO/KD验证抗体 KO-Validated GAPDH Mouse mAb

KO-Validated GAPDH Mouse mAb

品牌: 善本生物

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参数

UniProt ID: P04406
Reactive Species: Human,Mouse,Rat
Applications: WB,FCM
Conjugation: Unconjugated

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Applications

Reactive Species Human,Mouse,Rat
Applications WB,FCM
Recommended Dilution Western Blotting (WB): 1:4,000-1:20,000 Flow Cytometry (FCM): 1:200-1:2,000

Product Information

Product Name KO-Validated GAPDH Mouse mAb
Antibody Type Rabbit mAb
Immunogen A synthesized peptide derived from human GAPDH
Modification Unmodified
Host Rabbit
Clonality Monoclonal
Clone No. 24GB7870
Isotype IgG
Conjugation Unconjugated
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Conditions Store at -20 °C for one year.

Background Information

UniProt ID P04406
UniProt Entry Link https://www.uniprot.org/uniprotkb/P04406
Alternative Names GAPDH; Glyceraldehyde-3-Phosphate Dehydrogenase; GAPD; Peptidyl-Cysteine S-Nitrosylase GAPDH; EC 1.2.1.12; Epididymis Secretory Sperm Binding Protein Li 162eP; Oct1 Coactivator In S Phase, 38 Kd Component; Aging-Associated Gene 9 Protein; OCAS, P38 Component; HEL-S-162eP; EC 2.6.99.-; EC 1.2.1; G3PD
Gene ID 2597
Gene Name GAPDH
NCBI Gene Entry Link https://www.ncbi.nlm.nih.gov/gene/2597
Calculated MW 36 kDa
Observed MW 36 kDa

Pictures

Western blotting analysis using GAPDH antibody (Cat#71172). GAPDH expression in wild type (WT) and GAPDH knockout (KO) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with GAPDH antibody (Cat#71172, 1:20,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using GAPDH antibody (Cat#71172). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with GAPDH antibody (Cat#71172, 1:20,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of GAPDH expression in HepG2 cells using GAPDH antibody (Cat#71172, 1:2,000). Green, isotype control; red, GAPDH.

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KO-Validated GAPDH Mouse mAb

价格: ¥

600.00

UniProt ID: P04406
Reactive Species: Human,Mouse,Rat
Applications: WB,FCM
Conjugation: Unconjugated
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Western blotting analysis using GAPDH antibody (Cat#71172). GAPDH expression in wild type (WT) and GAPDH knockout (KO) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with GAPDH antibody (Cat#71172, 1:20,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

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