Western blotting analysis using CREB1 antibody (Cat#71152). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CREB1 antibody (Cat#71152, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). CREB1, cAMP responsive element binding protein 1.

Western blotting analysis using CREB1 antibody (Cat#71152). CREB1 expression in wild type (WT) and CREB1 knockout (KO) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with CREB1 antibody (Cat#71152, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Immunocytochemical staining of C2C12 cells with CREB1 antibody (Cat#71152, 1:1,000). Nuclei were stained blue with DAPI;CREB1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.